Data/hora: 14/05/2024 08:35:47

Biblioteca(s): 	Epagri-Sede.	
Data corrente: 	26/10/2018	
Data da última atualização: 	26/10/2018	
Autoria: 	SCHERER, R. F.; ANDRADE, A.; TULMANN NETO, A.; RIGO, G.	
Título: 	BANANA MUTAGENESIS IN VITRO: SENSITIVITY OF SHOOTS TO GAMMA RADIATION.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	In: FAO/IAEA INTERNATIONAL SYMPOSIUM ON PLANT MUTATION BREEDING AND BIOTECHNOLOGY, 2018, Viena, Áustria. Abstracts... Viena, Áustria: FAO/IAEA, 2018.	
Idioma: 	Inglês	
Conteúdo: 	Epagri is carrying out a project aimed at the induction of mutants for resistance to banana diseases by
irradiation of in vitro shoots of the cultivar SCS451 Catarina. The first step was the determination of
sensitivity to gamma rays using doses of 0; 12.5; 25; 37.5 and 50Gy. As it is known if in a shoot tip
after treatment with any mutagen the mutation may be induced in M1V1, however, there is the
occurrence of chimerism and further subcultures (M1V2, M1V3, M1V4, etc.) are needed to select and
isolate the stable mutants (periclinal chimeras or solid mutants). The shoots used were cultured using
the appropriate multiplication and growth media for banana. The experimental unit was composed of 3
flasks in M1V1 and 5 flasks in the others (M1V2 to M1V4) and 5 shoots were always inoculated in each
flask. The mean height and shoot regeneration rate (number of shoots formed at the number of
inoculated shoots) were evaluated at the end of each subculture (30-40 days interval). The treatments
that received the two highest doses (37,5 and 50Gy) presented the lowest regeneration rate (1,91 and
1,48, respectively) and the shortest shoot height (2,09 and 1,72 cm, respectively) at the end of M1V1. 
However, with the advance of the subcultures, the results of these treatments were like the others, so
that in M1V4 there were no significant differences. As in mutation breeding whenever possible it is
recommended to use more than one dose, based on the obtained results it was concluded that these two
doses (37,5 and 50Gy) can be used for the continuation of the research. For the continuation of the work
shoots were irradiated with each of these doses and the material is being led up to the M1V4 generation
for the selection.	
Palavras-Chave: 	gamma rays; genetic improvement; Musa spp; SCS451 Catarina.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_41406.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
Exemplares: 	Epagri-Sede - (102877-1) - UPC - PL - DD -  -  -  - DIS